Simultaneous determination of atropine and scopolamine in buckwheat and related products using modified QuEChERS and liquid chromatography tandem mass spectrometry

卷:218

Chen, Hongping ; Marin-Saez, Jesus ; Romero-Gonzalez, Roberto ; Garrido Frenich, Antonia

Univ Almeria

关键词: Atropine;    Scopolamine;    Buckwheat and related food;    Modified QuEChERS;    Liquid chromatography tandem mass spectrometry;    Transformation products;   

DOI  :  10.1016/j.foodchem.2016.09.075

学科分类：食品科学和技术

【 摘 要 】

A method was developed for the determination of atropine and scopolamine in buckwheat and related products. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction procedure was evaluated. Dispersive solid phase extraction (d-SPE) was studied as clean-up step, using graphitized black carbon (GBC) and primary secondary amine (PSA). The extract was diluted with water (50:50, v/v) prior to chromatographic analysis. The method was validated and recoveries (except chia samples spiked at 10 mu g/kg) ranged from 75% to 92%. Intra and inter-day precision was lower than or equal to 17%. The limit of quantification of atropine and scopolamine was 0.4 and 2 mu g/kg, respectively. Eight types of samples (buckwheat, wheat, soy, buckwheat flour, buckwheat noodle, amaranth grain, chia seeds and peeled millet) were analyzed. Target compounds were not found above the detection limits of the method, but three transformation products of scopolamine (norscopine, hydroscopolamine and dihydroxyscopolamine) were putative identified in the tested samples using high resolution mass spectrometry (Exactive-Orbitrap). (C) 2016 Elsevier Ltd. All rights reserved.