Diseases of Aquatic Organisms | |
Recombinant vaccines against infectious hematopoietic necrosis virus: production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials | |
Benjamin Simon1  Julian Thornton1  John Smit1  Peter Chiou1  Jo-Ann Leong1  John Nomellini1  Wade Bingle1  | |
关键词: Infectious hematopoietic necrosis virus; IHNV; Caulobacter crescentus; Fish virus; Rhabdovirus; Vaccines; S-layer; Type I secretion; T cell epitopes; | |
DOI : 10.3354/dao044017 | |
学科分类:生物科学(综合) | |
来源: Inter-Research | |
【 摘 要 】
ABSTRACT: We report the development of an IHNV vaccine produced by a new protein production system based on the bacterium Caulobacter crescentus. The subunit vaccines that were tested contain a 184 amino acid segment of the IHNV glycoprotein indifferent fusion arrangements with the C. crescentus S-layer protein. Relative percent survival of 26 to 34% was demonstrated in rainbow trout fry for a vaccine that contained the 184 amino acid segment of the IHNV glycoprotein fused to theC-terminal one-quarter of the S-layer protein. Inclusion of the universal mammalian T cell epitopes developed from the measles fusion protein or the tetanus toxin protein did not increase the effectiveness of the IHNV-G/S-layer recombinant protein.
【 授权许可】
Unknown
【 预 览 】
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RO201911300595625ZK.pdf | 199KB | download |