Recombinant vaccines against infectious hematopoietic necrosis virus: production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials

Diseases of Aquatic Organisms

Benjamin Simon¹  Julian Thornton¹  John Smit¹  Peter Chiou¹  Jo-Ann Leong¹  John Nomellini¹  Wade Bingle¹ 

关键词: Infectious hematopoietic necrosis virus;    IHNV;    Caulobacter crescentus;    Fish virus;    Rhabdovirus;    Vaccines;    S-layer;    Type I secretion;    T cell epitopes;   

DOI  :  10.3354/dao044017

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: We report the development of an IHNV vaccine produced by a new protein production system based on the bacterium Caulobacter crescentus. The subunit vaccines that were tested contain a 184 amino acid segment of the IHNV glycoprotein indifferent fusion arrangements with the C. crescentus S-layer protein. Relative percent survival of 26 to 34% was demonstrated in rainbow trout fry for a vaccine that contained the 184 amino acid segment of the IHNV glycoprotein fused to theC-terminal one-quarter of the S-layer protein. Inclusion of the universal mammalian T cell epitopes developed from the measles fusion protein or the tetanus toxin protein did not increase the effectiveness of the IHNV-G/S-layer recombinant protein.

【 授权许可】

Unknown