Efficacy of an infectious hematopoietic necrosis (IHN) virus DNA vaccine in Chinook Oncorhynchus tshawytscha and sockeye O. nerka salmon

Diseases of Aquatic Organisms

Kyle A. Garver¹  Scott E. LaPatra Gael Kurath¹ 

关键词: DNA vaccine;    Infectious hematopoietic necrosis virus;    IHNV;    Chinook salmon;    Sockeye salmon;    Cross-genogroup and intragenogroup challenge;   

DOI  :  10.3354/dao064013

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The level of protective immunity was determined for Chinook Oncorhynchus tshawytscha and sockeye/kokanee salmon (anadromous and landlocked) O. nerka following intramuscular vaccination with a DNA vaccine against the aquatic rhabdovirus, infectious hematopoietic necrosis virus (IHNV). A DNA vaccine containing the glycoprotein gene of IHNV protected Chinook and sockeye/kokanee salmon against waterborne or injection challenge with IHNV, and relative percent survival (RPS) values of 23 to 86% were obtained under a variety of lethal challenge conditions. Although this is significant protection, it is less than RPS values obtained in previous studies with rainbow trout (O. mykiss). In addition to the variability in theseverity of the challenge and inherent host susceptibility differences, it appears that use of a cross-genogroup challenge virus strain may lead to reduced efficacy of the DNA vaccine. Neutralizing antibody titers were detected in both Chinook and sockeyethat had been vaccinated with 1.0 and 0.1 µg doses of the DNA vaccine, and vaccinated fish responded to viral challenges with higher antibody titers than mock-vaccinated control fish.

【 授权许可】

Unknown