Sperm motility and acrossomal integrity in liquid boar semen dosis inoculated with Esecherichia coli and Staphylococcus aureus

Ciência Rural

Cardoso, Marisa Ribeiro de Itapema²  Bennemann, Paulo Eduardo¹  Bortolozzo, Fernando Pandolfo³  Wentz, Ivo⁴ 

[1] Universidade Federal do Rio Grande do Sul;Universidade Federal do Rio Grande do Sul, Porto Alegre;Universidade Federal de Santa Maria

关键词: semen;    acrosome;    Escherichia coli;    Staphylococcus aureus;   

DOI  :  10.1590/S0103-84782000000200019

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

Twelve ejaculates were collected in the most aseptic manner and distributed in seven treatments (control group T1). Semen were inoculated with S. aureus (T2, T3 e T4) or E. coli (T5, T6 e T7) in three concentrations (5 x 105, 5 x 106 and 5 x 107 CFU/ml). The sperm motility, the percentage of normal apical range (NAR), the pH and the number of colony unit former (CFU/ml) of bacteria, for 96 hours, were evaluated. The bacterial development was decreasing during the first 96 hours. Except for the treatment with the inoculation of 5 x 107 CFU of E. coli/ml, there was no significant effect of the bacteria on the sperm motility (p>0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 107 CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p<0.05). There was no correlation among the sperm motility, NAR, pH and CFU.

【 授权许可】

CC BY-NC