Effect of nematode Trichinella infection on glucose tolerance and status of macrophage in obese mice

Endocrine Journal

Kazuo Kajita¹  Zhiliang Wu²  Hiroyuki Morita¹  Yuzo Takahashi²  Yoshihiro Uno¹  Noriko Takahashi¹  Takahiro Yamada¹  Taro Usui¹  Tatsuo Ishizuka¹  Koichiro Taguchi¹  Takahide Ikeda¹  Yoshihiko Kitada¹  Hideyuki Okada¹  Kei Fujioka¹  Masahiro Yamauchi¹  Takuya Kudo¹  Isao Nagano²  Kazuki Furuya¹  Ichiro Mori¹  Takayuki Hanamoto¹ 

[1] Department of General Internal Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan;Department of Parasitology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan

关键词: Parasite infection;    Insulin resistance;    Adipose tissue inflammation;    Macrophage;   

DOI  :  10.1507/endocrj.EJ13-0312

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(19)Cited-By(2)We investigated the effect of Trichinella infection on glucose tolerance and (pro- or anti-inflammatory) macrophage status in adipose tissue.Ob/ob mice and high fat-fed mice (obesity model) and C57/BL mice (control mice) were orally infected with (infected group) or without (uninfected group) 400 Trichinella per mouse.Four weeks later, the mice were subjected to investigation, which showed that fasting plasma glucose levels decreased in the infected group of C57/BL and ob/ob mice.Glucose tolerance, evaluated with intraperitoneal GTT, improved in the infected group of ob/ob mice and high fat-fed mice compared with the uninfected groups.Additional assay included anti-inflammatory macrophage (M2) markers and pro-inflammatory macrophage (M1) markers, with the aim to explore the effect of Trichinella infection on adipose tissue inflammation, since our previous study identified anti-inflammatory substances in secreted proteins by Trichinella.The result showed that mRNA levels of M2 markers, such as CD206, arginase and IL-10, increased, whereas M1 markers, such as CD11c, iNOS and IL-6, decreased in the stromal vascular fraction (SVF) isolated from epididymal fat in ob/ob mice.Residential macrophages obtained from the peritoneal lavage exhibited lower M1 markers and higher M2 markers levels in the infected group than in the uninfected group.Trichinella infection increases the ratio of M2/M1 systemically, which results in an improvement in pro-inflammatory state in adipose tissue and amelioration of glucose tolerance in obese mice.

【 授权许可】

Unknown