In Vitro and In Vivo Effects of Xanthorrhizol on Human Breast Cancer MCF-7 Cells Treated With Tamoxifen

Journal of Pharmacological Sciences

Chun-Ju Chang⁶  Nattanant Noomhorm⁵  Che-Sheng Wen³  Jen-Hwey Chiu⁵  Jiun-Liang Chen⁷  Wei-Shone Chen¹  Yi-Ming Shyr⁴  Ling-Ming Tseng⁴  Jir-You Wang² 

[1] Division of Colorectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taiwan;Department of Orthopedics, Taipei Veterans General Hospital, Taiwan;Department of Orthopedics, Cheng-Hsin General Hospital, Taiwan;Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taiwan;Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taiwan;Department of Food Science, National Taiwan Ocean University, Taiwan;School of Traditional Chinese Medicine, Chang-Gung University, Taiwan

关键词: breast cancer;    tamoxifen;    interaction;    xanthorrhizol;   

DOI  :  10.1254/jphs.14024FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(29)Cited-By(2)This study investigated the herb–drug interaction of xanthorrhizol and tamoxifen in human breast cancer cells. Using MCF-7 cell line as an in vitro model, the herb–drug interaction between xanthorrhizol and tamoxifen was measured by MTT assay, luciferase reporter assay, and cell cycle analysis. The effects of xanthorrhizol on growth/autophagy related signaling were determined by immunostaining, western blotting, and real time RT-PCR. Additionally, the in vivo effect of xanthorrhizol and tamoxifen on athymic nude mice implanted with MCF-7 cells was evaluated. When MCF-7 cells were co-treated with tamoxifen and xanthorrhizol, there were no significant changes in terms of cell number, luciferase activity, percentage S-phase cells and LC3-II expression. However, using the MCF-7 implanted nude mice model, it was possible to detect significantly increased tumor volumes, a larger tumor size, and increased protein expression of P38 and P27(Kip1) in the xanthorrhizol + tamoxifen group compared to the tamoxifen-alone group. It can be concluded that while there is no significant herb–drug interaction between xanthorrhizol and tamoxifen in vitro, there is such an interaction in tumor-bearing mice, which provides important information that affects breast cancer treatment translational research.

【 授权许可】

Unknown