Effects of Chlorpromazine, Pentoxifylline and Dexamethasone on mRNA Expression of Lipopolysaccharide-Induced Inflammatory Cytokines in Bovine Peripheral Blood Mononuclear Cells

Journal of Veterinary Medical Science

Nobuhiro TERASAKI⁶  Masateru KOIWA²  Hiromichi OHTSUKA⁷  Tomo-o YOSHINO⁵  Satoru KON-NAI³  Shigeo FUKUDA⁵  Yoshio KIKU⁵  Hiroshi SATO⁶  Thomas J. ROSOL¹  Yuichi YOKOMIZO⁴  Hiroyuki OKADA⁵  Hironori MATSUZAWA⁶ 

[1] Department of Veterinary Biosciences, The Ohio State University;Department of Veterinary Teaching Hospital, Large Animal Clinical Center, School of Veterinary Medicine, Rakuno Gakuen University;Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University;National Institute of Animal Health;Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University;Department of Veterinary Internal Medicine, School of Veterinary Medicine, Rakuno Gakuen University;Department of Veterinary Internal Medicine, School of Veterinary Medicine, Kitasato University

关键词: bovine;    cytokine;    lipopolysaccharide;   

DOI  :  10.1292/jvms.64.723

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(10)The effects of chlorpromazine (CPZ), pentoxifylline (PTX) and dexamethasone (DEX) on mRNA expression of lipopolysaccharide (LPS)-induced proinflammatory cytokines were examined in bovine peripheral blood mononuclear cells (PBMCs) in vitro. The expression of inflammatory cytokine mRNAs was analyzed by RT-PCR and Southern blot hybridization in bovine PBMCs. CPZ and DEX decreased the expression of cytokine mRNA (such as interleukin-1β and tumor necrosis factor-α) after stimulation with LPS in a dose-dependent manner. However, pretreatment with PTX had no inhibitory effect on the mRNA expression of proinflammatory cytokines. These results indicated that pretreatment with CPZ and DEX might be effective to reduce the production of LPS-induced inflammatory cytokines in bovine PBMCs in vitro.

【 授权许可】

Unknown