Effects of Various Selective Phosphodiesterase Inhibitors on Relaxation and Cyclic Nucleotide Contents in Porcine Iris Sphincter

Journal of Veterinary Medical Science

Yasushi HARA²  Kazumasa SHIMIZU¹  Yasuji HARADA²  Takuya YOGO²  Yoshinori NEZU²  Masahiro TAGAWA²  Norimoto URAKAWA¹  Takeharu KANEDA¹ 

[1]Laboratory of Veterinary Pharmacology, Nippon Veterinary and Life Science University

[2]Laboratory of Veterinary Surgery, Nippon Veterinary and Life Science University

关键词: cAMP;    cGMP;    iris sphincter;    PDE inhibitor;    smooth muscle;   

DOI  :  10.1292/jvms.001449

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(1)The effects of various selective phosphodiesterase (PDE) inhibitors on muscle contractility and cyclic nucleotide contents in porcine iris sphincter were investigated. Forskolin and sodium nitroprusside inhibited carbachol (CCh)-induced contraction in a concentration-dependent manner. Various selective PDE inhibitors, vinpocetine (type 1), erythro -9-(2-hydroxy-3-nonyl)adenine (EHNA, type 2), milrinone (type 3), Ro20-1724 (type 4) and zaprinast (type 5), also inhibited CCh-induced contraction in a concentration-dependent manner. The rank order of potency of IC50 was zaprinast > Ro20-1724 > EHNA ≥ milrinone > vinpocetine. In the presence of CCh (0.3 μM), vinpocetine, milrinone and Ro20-1724 increased cAMP, but not cGMP, contents. In contrast, zaprinast and EHNA both increased cGMP, but not cAMP, contents. This indicates that vinpocetine-, milrinone- and Ro20-1724-induced relaxation is correlated with cAMP, while EHNA- and zaprinast- induced relaxation is correlated with cGMP in porcine iris sphincter.

【 授权许可】

Unknown