PDE4 and PDE5 regulate cyclic nucleotide contents and relaxing effects oncarbachol-induced contraction in the bovine abomasum

Journal of Veterinary Medical Science

Kazumasa SHIMIZU¹  Yuuki KIDO¹  Tsuyoshi TAJIMA¹  Norimoto URAKAWA¹  Takeharu KANEDA¹ 

[1]Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 7�?1 Kyonan-cho 1-chome, Musashino, Tokyo 180�?8602, Japan

关键词: abomasum;    cAMP;    cGMP;    PDE inhibitor;   

DOI  :  10.1292/jvms.14-0248

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(1)The effects of variousselective phosphodiesterase (PDE) inhibitors on carbachol (CCh)-induced contraction in thebovine abomasum were investigated. Various selective PDE inhibitors, vinpocetine (type 1),erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA, type 2), milrinone (type 3), Ro20-1724 (type4), vardenafil (type 5), BRL-50481 (type 7) and BAY73-6691 (type 9), inhibited CCh-inducedcontractions in a concentration-dependent manner. Among the PDE inhibitors, Ro20-1724 andvardenafil induced more relaxation than the other inhibitors based on the data for theIC50 or maximum relaxation. In smooth muscle of the bovine abomasum, weshowed the expression of PDE4B, 4C, 4D and 5 by RT-PCR analysis. In the presence of CCh,Ro20-1724 increased the cAMP content, but not the cGMP content. By contrast, vardenafilincreased the cGMP content, but not the cAMP content. These results suggest thatRo20-1724-induced relaxation was correlated with cAMP and that vardenafil-inducedrelaxation was correlated with cGMP in the bovine abomasum. In conclusion, PDE4 and PDE5are the enzymes involved in regulation of the relaxation associated with cAMP and cGMP,respectively, in the bovine abomasum.

【 授权许可】

Unknown