Velvet-bean nucleopolyhedrovirus persistence after different rainfall intensities

Ciência Rural

Bortolotto, Orcial Ceolin¹  Cunha, Fabiane²  Bueno, Adeney de Freitas¹  Monteiro, Talita³  Ramos, Vânia Maria²  Pessoa, Valter² 

[1] Embrapa Soja, Londrina, Brasil;Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, Brasil;Universidade Estadual do Oeste do Paraná, (UNIOESTE)

关键词: baculovirus;    abiotic factors;    Glycine max;    Anticarsia gemmatalis;    entomopathogen;   

DOI  :  10.1590/S0103-84782014000100002

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The persistence of the velvet-bean nucleopolyhedrovirus (AgMNPV) produced raw (macerated caterpillars) and lyophilized (commercial product) was evaluated after spraying in soybean plants followed by different simulated rainfall rates (0; 10; 20; 30mm) at 30mm hour-1 rate. Rainfall was simulated in greenhouse through micro sprinkler irrigation. After simulated rainfall, soybean leaves were offered to caterpillar in laboratory conditions at zero, three, six and nine days after spraying (DAS). Soybean plants were kept in greenhouse protected from rainfall. Trial was carried out in complete randomized design, under controlled conditions (RH 70%±10%, 25±2°C, 14h photophase) in a factorial 4 (rainfall rates) x2 (formulations, raw and lyophilized) with four replicates of 30 caterpillars. Results indicate that rainfall rates up to 30mm, in 60 minutes, do not decrease caterpillar mortality caused by AgMNPV regarding to both tested baculovirus formulations, raw and lyophilized virus, when leaves were offered to caterpillars at zero DAS. However, virus production (raw or lyophilized) impacted its persistence. Six DAS, mortality rate was higher than 90% when the virus was applied raw and lower than 50% when it was applied lyophilized. These results suggest that both raw and lyophilized AgMNPV efficacy is not impaired by 30-mm rainfalls at 30mm hour-1 rate. However, raw virus was more efficient in a long term evaluation since it had longer persistence on soybean leaves after being water washed.

【 授权许可】

CC BY-NC