Dysfunction of Erythropoietin-Producing Interstitial Cells in the Kidneys of ICR-derived Glomerulonephritis (ICGN) Mice

Journal of Veterinary Medical Science

Chinatsu SAKATA⁵  Noboru MANABE¹  Misuzu YAMAGUCHI-YAMADA⁵  Sayuri ANAN⁵  Minako KISO⁵  Toshiharu MORI⁵  Yoshie YAMAMOTO²  Masaya NAGAO⁴  Yasufumi GOTO⁵  Atsuo OGURA³ 

[1] Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo;Department of Veterinary Sciences, National Institute of Infectious Diseases;Bioresource Center, RIKEN;Laboratory of Biosignals and Response, Department of Applied Molecular Biology, Kyoto University;Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University

关键词: amplified in situ hybridization;    anemia with chronic renal disorder (CRD);    erythropoietin (EPO)-producing cell;    hereditary nephrotic ICR-derived glomerulonephritis (ICGN) mouse;    kidney;   

DOI  :  10.1292/jvms.67.891

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(37)Cited-By(3)Anemia is a major secondary symptom in chronic renal disorder (CRD), but the precise cause of insufficient production of erythropoietin (EPO) remains unclear owing to the controversial localization of EPO-producing cells in the kidneys. The ICR-derived glomerulonephritis (ICGN) mouse, a new hereditary nephrotic mouse, is an appropriate model of anemia associated with CRD. By using an amplified in situ hybridization technique, we detected and counted the renal EPO-producing cells under both normoxic and hypoxic conditions. The expression levels of renal EPO mRNA were quantified and oxygen gradients were also assessed immunohistochemically. Amplified in situ hybridization clarified that EPO-producing cells were peritubular interstitial cells in the middle region of renal cortex in both ICR and ICGN mice. Hypoxia (7% O2) induced low oxygen tension in proximal tubular epithelial cells of renal cortex, and increased the expression of EPO mRNA and the number of EPO-producing cells in both ICR and ICGN mice. However, hypoxia did not increase the serum EPO levels in ICGN mice. The ICGN mouse is a good model for anemia associated with CRD, and the suppression of EPO protein production in the renal EPO-producing cells is considered to be a potential cause of anemia associated with CRD.

【 授权许可】

Unknown