Oxidative Damage to the Membrane of Canine Erythrocytes with Inherited High Na, K-ATPase Activity

Journal of Veterinary Medical Science

Tomohiko AMAYA¹  Eri OGAWA¹  Takahiro YAGI¹  Atsushi KAWAKAMI¹  Reiji TAKAHASHI¹  Hiroshi FUJISE¹ 

关键词: canine HK cell;    glutathione;    lipid peroxidation;    malondialdehyde;    β-acetylphenylhydrazine;   

DOI  :  10.1292/jvms.54.57

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(31)Cited-By(3)Oxidative damage to the membrane in canine erythrocytes with inherited high Na, K-ATPase activity (HK cells) was compared with that in normal canine cells (LK cells). When 30 mM β-acetylphenylhydrazine (APH) was applied to HK and LK cells, lipid peroxidation and hemoglobin denaturation occurred. Lipid peroxidation determined from malondialdehyde (MDA) formation was significantly lower in HK than in LK cells so far as endogenous glutathione (GSH) concentration was maintained at appropriate levels. With the depletion of GSH, MDA formation was accelerated and difference between HK and LK cells was not significant. Denatured hemoglobin bound to the membrane protein was less in HK than in LK cells. During incubation with APH, osmotic fragility increased markedly in LK cells, while HK cells showed very little change. The amounts of total lipid, total and free cholesterol, glycolipid, phospholipid and fatty acids were essentially the same in both cell types. Fatty acid compositions showed very small differences. The membrane of HK cells thus appear to have greater protection against oxidative damage induced by APH, owing to the presence of excess GSH in HK cells. The capability of HK cells to withstand oxidative damage would not be due to differences in membrane lipid compositions.

【 授权许可】

Unknown