Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions

Colombia Médica

José Henry Osorio¹  Morteza Pourfarzam² 

[1] Universidad de Caldas;Royal Victoria Infirmary

关键词: Carnitine;    Acylcarnitines;    Acyl-CoA;    Tandem mass spectrometry;    Red blood cells;    Metabolism.;   

DOI  :  

学科分类：医学（综合）

来源: Universidad del Valle * Facultad de Salud / Universidad del Valle, Faculty of Health

【 摘 要 】

Objective:To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution.Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis.Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05).Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.

【 授权许可】

Unknown