A Simple Technique for Isolating Healthy Heart Cells from Mouse Models

The Journal of Physiological Sciences

Takao Shioya¹ 

[1] Department of Physiology, Faculty of Medicine, Saga University

关键词: mouse;    cardiac myocyte;    contraction;    action potential;    ionic current;   

DOI  :  10.2170/physiolsci.RP010107

学科分类：生理学

来源: Springer

【 摘 要 】

References(38)Cited-By(22)Single heart cells of mouse models provide powerful tools for heart research. However, their isolation is not easy, and it imposes a significant bottleneck on their use in cellular studies of the heart. Aiming to overcome this problem, this report introduces a novel technique that reproducibly isolates healthy heart cells from mouse models. Using simple devices that ensure easy handling and the rapid aortic cannulation of a small mouse heart, cell isolation was done under physiological conditions without using the “KB” medium or 2,3-butanedione monoxime (BDM). The isolated cells consistently had a healthy appearance and a high viability of 75 ± 5% (mean ± SD) in Tyrode solution containing 1.8 mM Ca2+. After 8 h of storage at 37°C, they still had a viability of 45 ± 12%. The cells showed normal contraction properties when field-stimulated, and they generated normal action potentials and membrane currents under the whole-cell clamp condition. The β-adrenergic signal transduction of the cells was also normal when it was examined with the isoproterenol enhancement of the L-type Ca2+ current.

【 授权许可】

Unknown