Serofendic Acid Protects Against Myocardial Ischemia–Reperfusion Injury in Rats

Journal of Pharmacological Sciences

Takeshi Kimura¹  Akinori Akaike²  Toshiaki Kume²  Yasuhiko Izumi²  Masashi Kato¹  Masaharu Akao¹  Moritake Iguchi¹  Tadaaki Ioroi² 

[1] Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Japan;Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

关键词: serofendic acid;    myocardial ischemia;    reperfusion;    mitochondrial ATP-sensitive potassium channel;    cardiac myocyte;   

DOI  :  10.1254/jphs.14139FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(38)Cited-By(2)We previously reported that serofendic acid, a lipophilic extract of fetal calf serum, protects against oxidative stress in primary culture of neonatal rat cardiomyocytes. However, the effect of serofendic acid on myocardial ischemia–reperfusion injury in vivo is yet to be determined. In the present study, we investigated the effect of intravenous administration of serofendic acid on ischemia–reperfusion injury induced by transient occlusion of the left coronary artery in rats. The rat heart was subjected to 25-min ischemia followed by 2-h reperfusion. Bolus intravenous administration of serofendic acid (1 �? 10 mg/kg) given twice reduced the infarct volume in a dose-dependent manner. The protective effect of serofendic acid was abolished by pretreatment with 5-hydroxydecanoate, a blocker of mitochondrial ATP-sensitive potassium channels. For further testing of the protective effect of serofendic acid at the subcellular level, we monitored mitochondrial membrane potential (MMP) in individual cells using real-time two-photon imaging of Langendorff-perfused rat heart. A 25-min no-flow ischemia, followed by reperfusion caused progressive MMP loss. Serofendic acid significantly reduced the number of cells undergoing MMP loss. These results suggest that serofendic acid protected cardiac myocytes against myocardial ischemia–reperfusion injury by preserving the functional integrity of mitochondria.

【 授权许可】

Unknown