EFFECT OF THE ADDITION OF FSH AND LH IN MEDIUM ON THE MATURATION AND THE EMBRYONIC DEVELOPMENT in vitro OF OOCYTES OF SOW

Revista de Salud Animal

Hernández Pichardo, J.E¹  Fernández Reyes, F¹  Rosales Ávila, María del Rocio² 

[1] Autónoma Metropolitana-Xochimilco (UAM-X), México;Clínica Privada

关键词: pig;    oocyte;    embryo;    maturation in vitro;    fertilization in vitro    INTRODUCCIÓNLos ovocitos son células altamente especializadas y las únicas dependientes de la introducción de ácido desoxirribonucleico (ADN) externo;    proveniente del espermatozoide para poder llevar a cabo las siguientes fases de desarrollo (1);   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

One thousand twenty five oocytes from sow ovaries, obtained in slaughter houses were recovered. From them, 165 (treatment 1 ) were matured with 075 U.I. of FSH and 0.75 U.I. of LH, and 125 (treatment 2 ) with 1.5 U.I. of FSH and 1.5 U.I. of LH. Maturation was evaluated by fixation with alcohol-acetic acid and stained with acetic orcein. There was a 40% and 39% of maturated oocytes from the treatments 1 and 2 respectively, without significant statistical differences among them. In the two treatments for in vitro fertilization, the maturation of oocytes was evaluated by polar body observation. They were divided into two groups: one having 155maturated with the first treatment and the other having 137 oocytes with the second treatment, which corresponded to a 42.1% and 37.3% respectively . As result of in vitro fertilization, 122 embryos were obtained in their different stages, reaching 76 with the first treatment and 46 with the second one, for a 49% and 33.5% (a different fom b p <0.05) respectively . It is concluded that even though FSA and LH supplement to the maturation medium TCM-199 (in vitro) did not significantly affect such maturation, in the development of embryos, there was a higher percentage of morulas and embryonic development with the use of 0.75U.I. of FSH and 0.75 0.75U.I: of LH.

【 授权许可】

CC BY-NC