EIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar Wash | Science Publications

American Journal of Animal and Veterinary Sciences

R C. Leite¹  Jenner K.P.D. Reis¹  Pedro M.C. Motta¹  Helen L.D. Puerto¹  Marcos B. Heinemann¹  Elizangela M.D. Santos¹ 

关键词: Diagnosis;    EIA;    EIAV;    nPCR and PBMCs;   

DOI  :  10.3844/ajavsp.2010.180.182

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectiousdisease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experimentwas compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) andBronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samplesisolated from PBMCs and BAW of naturally infected animals were used for a nested PCR amplifying a408 bp gag gene fragment region. Equine Dermal cells (ED) infected and non infected by EIAV wasused as nested PCR negative and positive control. Horses

【 授权许可】

Unknown