Quantitative Diversity of 67kDa Protective Antigen among Serovar 2 Strains of Erysipelothrix rhusiopathiae and Its Implication in Protective Immune Response

Journal of Veterinary Medical Science

Yukio SHIMIZU¹  Eiji OISHI¹  Hisae NAKAMURA¹  Takashi KITAJIMA¹  Kenji ODA¹  Katsuhiko AMIMOTO¹  Akihiro IZUMIDA¹  Satoshi UI¹  Shigeji KATAYAMA¹ 

[1] Division of Veterinary Microbiology,Kyoto Biken Laboratories,24-16 Makishima-cho,Uji,Kyoto 611-0041,Japan

关键词: bacterin;    Erysipelothrix rhusiopathiae;    monoclonal antibody;    67kDa protective antigen;   

DOI  :  10.1292/jvms.62.1073

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(3)Mouse monoclonal antibodies(MAbs), raised against the NaOH−extracted antigen of Erysipelothrix rhusiopathiae strain Kyoto(serovar 2), recognized two different epitopes on a single protein of molecular weight 67 kDa.The MAbs were classified as protective or non−protective against strain Fujisawa(serovar 1).In immunoblotting analysis using the MAbs, fifteen wild strains were shown to contain different amounts of 67 kDa protective antigen.Each formalin−killed whole cell vaccine(bacterin)prepared from the fifteen wild strains conferred different levels of protection against strain Fujisawa in mice.Bacterins prepared from wild strains with larger amounts of 67 kDa protective antigen tended to give high levels of antigen−specific antibody and better protection to mice.These results indicate that the amount of 67 kDa protective antigen which influences the induction of protective immune responses may vary substantially among the strains of E.rhusiopathiae(serovar 2).

【 授权许可】

Unknown