PRODUCTION AND SEROLOGIE EVALUATION OF A BACTERIN AGAINST BOVINE LEPTOSPIROSIS

Revista MVZ Córdoba

Arias P, Janeth²  Fernández L, Cindy²  Gutiérrez, Benito¹  Sánchez, Edna¹ 

[1] Pontificia Universidad Javeriana;Departamento de Microbiología, Bogotá

关键词: Leptospira;    bovine;    bacterin;    vaccine INTRODUCCIÓNLa leptospirosis es una de las zoonosis más difundidas en el mundo;    constituye un grupo de enfermedades bacterianas que determinan una infección aguda generalizada;   

DOI  :  

学科分类：兽医学

来源: Universidad de Cordoba * Facultad de Medicina Veterinaria y Zootecnia

【 摘 要 】

Objective. To produce a bacterin against bovine leptospirosis.Materials and methods. The production of bacterin was made using the serovares: L. pomona, L grippotyphosa, L. hardjobovis, L. hardjoprajitno, L. icterohaemorrhagiae and L. canicola with concentrations between 1x108 and 1x1010 cells/ml. Leptospiras were immobilized with formaldehyde and adsorption of aluminum hydroxide to formulate the vaccine. Final concentration of each serovar was of 1x108 Leptospiras/ml. A population of 20 healthy calves bleeding before and after vaccination was immunized to analyze serum evaluation of the biological product to make titrations of serums by means of micro-agglutination technique and this way to determine antibodies production generated by bacterin.Results. L. icterohaemorrhagiae was the serovar with higher titres of antibodies followed by L. canicola. Serums from bleeding made 15 days before revaccination were those with higher number of antibodies for all serovars except for L. grippotyphosa that was the only one that did not stimulate antibodies production in vaccinated animals. Conclusions. The bacterin was able to induce a humoral response in bovines, however, L. grippotyphosa was not immunogen compared with other serovars that participated in the vaccine.

【 授权许可】

Unknown