Establishment and Characterization of a New Cell Line Derived from Feline Mammary Tumor

Journal of Veterinary Medical Science

Junya SUGAHARA³  Janet S. MULEYA³  Sanenori NAKAMA³  Yasuho TAURA¹  Munekazu NAKAICHI³  Tomoaki MURATA² 

[1]Department of Veterinary Hospital, Yamaguchi University

[2]Laboratory Animals, School of Medicine, Yamaguchi University

[3]Department of Veterinary Surgery, Yamaguchi University

关键词: adenocarcinoma;    cell line;    feline;    mammary gland;    telomerase;   

DOI  :  10.1292/jvms.60.931

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(8)A new cell line designated FRM was established from pleural effusion of a 13-year-old female cat with mammary adenocarcinoma. The cell line exhibited irregular round and polygonal shaped epithelial cells and demonstrated cell growth in a monolayer fashion with a doubling time of 22.4 hr. It possessed a modal chromosome number of 79. The immortality of this cell line was demonstrated using the TRAP assay which revealed a high telomeric activity of these cells. Scatchard analysis revealed quite low levels of estrogen receptors in both tumor mass produced in nude mice and FRM cells. Subcutaneous transplantation of the cells produced localized palpable masses in athymic nude mice within two weeks. This cell line may provide a good model for in vivo and in vitro studies on feline mammary tumors.

【 授权许可】

Unknown