Loop-Mediated Isothermal Amplification (LAMP) for Detection of Campylobacter jejuni and C. coli in Thai Children with Diarrhea

Japanese Journal of Infectious Diseases

Pattara Khamrin¹  Hiroshi Ushijima⁵  Niwat Maneekarn¹  Satoshi Hayakawa⁵  Wataru Yamazaki⁴  Ngan Thi Kim Pham⁵  Nuthapong Ukarapol²  Shoko Okitsu⁵  Tipachan Kongsricharoern³  Quang Duy Trinh⁵  Shihoko Komine-Aizawa⁵ 

[1]Department of Microbiology, Faculty of Medicine, Chiang Mai University

[2]Department of Pediatrics, Faculty of Medicine, Chiang Mai University

[3]Pediatric Hematology Unit, Nakornping Hospital

[4]Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

[5]Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine

关键词: Campylobacter;    children;    diarrhea;    LAMP;    Thailand;   

DOI  :  10.7883/yoken.JJID.2014.450

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(11)Cited-By(2)Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hipO gene (in C. jejuni) or the ask gene (in C. coli). The results suggest that the prevalence of Campylobacter infection among Thai children with diarrhea is 8.6%, and C. jejuni is the most prevalent species.

【 授权许可】

Unknown