Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos

Endocrine Journal

HISAHIKO HIROI¹  MIKIO MOMOEDA¹  HIROTAKA MATSUMI¹  OSAMU TSUTSUMI¹  MASAMI MURAMATSU²  FUJIKO TSUCHIYA¹  YUJI TAKETANI¹  SATOSHI INOUE² 

[1] Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo;Department of Biochemistry, Saitama Medical School

关键词: Estrogen receptor subtypes;    Estrogen-responsive finger protein;    Preimplantation embryos;    Mouse;   

DOI  :  10.1507/endocrj.46.153

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(31)Cited-By(19)In hope of understanding possible roles of estrogen during early embryogenesis, we examined the expression of both estrogen receptor α (ERα) and ERβ, a recently cloned novel subtype, in mouse oocytes and preimplantation embryos by means of reverse transcription polymerase chain reaction (RT-PCR). To investigate whether estrogen actually exerts its action, we further determined the expression of efp (estrogen-responsive finger protein), a newly characterized estrogen responsive gene belonging to the RING finger family. ERα mRNA was detected in whole ovaries, cumulus-oocyte complexes, denuded oocytes, 2-cell and 4-cell embryos, whereas it was undetected in 8-cell embryos. Interestingly it reappeared in morulae and blastocysts. ERβ mRNA was detected similarly to ERα except for the absence of ERβ mRNA in morulae. The efp mRNA was detected in whole ovaries, cumulus-oocyte complexes, 4-cell embryos, morulae and blastocysts. The stage specific expression of ERα and ERβ along with detection of the product of the estrogen responsive gene in early preimplantation embryos may indicate the possible physiological roles of estrogen in early embryogenesis.

【 授权许可】

Unknown