Immunohistolocalization of Carbonic Anhydrase Isozyme (CA-VI) in Bovine Mammary Glands

Journal of Veterinary Medical Science

Hajime AMASAKI²  Nobutsune ICHIHARA³  Masao ASARI³  Tamae KASUYA³  Kumi MATSUI¹  Toshiho NISHITA⁴  Etsuko SUSAKI³ 

[1] Department of Biology, School of Education, Waseda University;Department of Veterinary Anatomy, Nippon Veterinary and Animal Science University;Department of Anatomy, School of Veterinary Medicine, Azabu University;Department of Physiology, School of Veterinary Medicine, Azabu University

关键词: bovine mammary gland;    CA-VI;    immunohistochemical localization;   

DOI  :  10.1292/jvms.65.1167

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(7)The localization of bovine carbonic anhydrase isozyme VI (CA-VI) was examined immunohistochemically in bovine mammary glands during early lactation period (after 2-3 days of postpartum) and dry period (at about 2 months preparturition in adults), and young calves (at 30 and 150 days after birth) using specific CA-VI antiserum. The immunoreaction for anti-CA-VI antiserum was very weak in the mammary glands in young (prepubescent) calves. In dry period, CA-VI was also weakly expressed in secretory epithelial (acinar) and ductal cells. In contrast, the reaction was intense in mammary gland cells in early lactation period. Dot blotting analysis indicated that anti-CA-VI reacted positively to beastings and mature saliva, but weakly or not at all to milk during the dry period or calf saliva, respectively. The intense expression of CA-VI in the mammary glands in early lactation period might compensate for low levels of secretion from functionally and structurally immature salivary glands in young calves.

【 授权许可】

Unknown