Immunohistolocalization and Gene Expression of Secretory Carbonic Anhydrase Isoenzyme CA-VI in Canine Nasal Cavity

Journal of Veterinary Medical Science

Hajime AMASAKI¹  Nobutsune ICHIHARA²  Yosuke SUGIURA²  Masao ASARI²  Toshiho NISHITA³  Masaru MURAKAMI⁴ 

[1] Department of Veterinary Anatomy, Nippon Veterinary and Life Science University;Department of Anatomy, School of Veterinary Medicine, Azabu University;Department of Physiology, School of Veterinary Medicine, Azabu University;Department of Molecular Biology, School of Veterinary Medicine, Azabu University

关键词: canine nasal cavity;    CA-VI;    immunohistochemistry;    mRNA;   

DOI  :  10.1292/jvms.70.1037

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(36)Cited-By(4)Immunolocalization of the secretory form of carbonic anhydrase isoenzyme, CA-VI were studied using a specific canine CA-VI antiserum, and CA-VI mRNA signals were also investigated using the reverse-transcriptase polymerase chain reaction (RT-PCR) in canine nasal mucosal epithelia and glands. Immunoreactivity to CA-VI was positive throughout the mucosal epithelial cells and in the cytoplasm of serous acinar and ductal epithelial cells of the nasal mucosa and glands, including the vestibule of the nose, but the mucous acinar cells of the glands were immunonegative. We detected CA-VI gene transcripts in the same regions as the CA-VI immunoreactivity. The physiological roles of CA-VI in the nasal mucosal epithelium and glands might maintain bicarbonate levels in nasal secretions and protect the mucosa against acid.

【 授权许可】

Unknown