Genetic markers of pathogenicity in Yersinia enterocolitica O: 3 isolated from healthy pigs from Rio de Janeiro

Pesquisa Veterinária Brasileira

Almeida, Alzira M. P.¹  Leal, Tereza C. A.²  Leal, Nilma C.¹ 

[1] Fundação Oswaldo Cruz, Recife

关键词: Yersinia enterocolitica;    swine;    genetic markers;    pathogenicity.;   

DOI  :  10.1590/S0100-736X1997000100004

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

.- Leal T.C.A., Leal N.C. & Almeida A.M.P. 1997. [Genetic markers of pathogenicity in Yersinia enterocolitica O:3 isolated from healthy pigs from Rio de Janeiro.] Marcadores de patogenicidade em Yersinia enterocolitica O:3 isoladas de suínos do Rio de Janeiro. Pesquisa Veterinária Brasileira 17(1):19-24. Depto Microbiologia, CPqAM-FIOCRUZ-MS, Cx. Postal 7472, Cidade Universitária, Recife, PE 50670-420, Brazil.Sixteen Yersinia enterocolitica serotype O:3 strains, isolated from pigs from Rio de Janeiro, have been analyzed for genetic and phenotypic markers of pathogenicity. It was observed that only 6 strains harbored the pYV (+70 kb) plasmid and one strain harbored a small cryptic plasmid of about 8.6 kb. Accordingly only strains harboring pYV were calcium dependent in the MOX medium at 370C. Twelve strains showed pesticin sensitivity and the esculin reaction was negative in 13 strains. PCR analysis of pathogenicity genes using specific primers showed the presence of the ail gene in 14 strains, the irp2 gene in one and the psaA in none. Most of the strains were resistant to ampicillin and carbenicillin, although they were susceptible to sulfazotrin and cefoxitin. For chloramphenicol, tetracycline, kanamycin, gentamicin and nalidixic acid the results varied among the strains.INDEX

【 授权许可】

CC BY-NC