Characteristics of Major Structural Protein Coding Gene and Leader-Body Sequence in Subgenomic mRNA of Porcine Reproductive and Respiratory Syndrome Virus Isolated in Japan

Journal of Veterinary Medical Science

Masatake MURAMATSU¹  Toru KANNO¹  Shigeo YAMAGUCHI¹  Yosuke MURAKAMI¹  Akito SAITO¹ 

关键词: junction sequence;    nucleotide sequence;    porcine reproductive and respiratory syndrome;   

DOI  :  10.1292/jvms.58.377

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(6)Nucleotide sequence, 1713nt in length, of porcine reproductive and respiratory syndrome virus (PRRSV) isolated in Japan was determined. The sequence encompassed 3 overlapping open reading frames (ORFs), ORF5 to ORF7. These ORFs encodes major structural proteins of PRRSV. The deduced amino acid sequence of each ORF showed higher than 87.5% identity with an American isolate, and lower (54.6 to 80.5%) identity with an European isolate. This result supported a previous report about antigenic characteristics of the EDRD-1 strain. Leader-body junction sequence in subgenomic mRNA of the EDRD-1 strain was determined by sequencing cDNA clones of subgenomic RNAs. A common sequence motif of 5 nucleotide, represented by UA(A/G)CC, was identified as the junction sequence.

【 授权许可】

Unknown