Isolation of Porcine Respiratory Coronavirus from Pigs Affected with Porcine Reproductive and Respiratory Syndrome

Journal of Veterinary Medical Science

Masanori KUBO¹  Shunji YAMADA¹  Matsuyoshi KANEKO¹  Mitsugu SHIMIZU¹  Osamu KAMOGAWA¹  Yutaka TOMITA¹ 

关键词: porcine reproductive and respiratory syndrome;    porcine respiratory coronavirus;    PRCV;   

DOI  :  10.1292/jvms.58.385

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(4)Four cytopathogenic viruses were isolated in CPK cells derived from porcine kidneys from tonsils and lungs of 3 of 15 pigs affected with porcine reproductive and respiratory syndrome virus. Physicochemically and morphologically, the isolates were similar to a coronavirus. The isolates were not distinguished from transmissible gastroenteritis virus (TGEV) by a neutralization test using polyclonal antibodies, but differentiated from TGEV by monoclonal antibodies capable of discriminating between TGEV and porcine respiratory coronavirus (PRCV), indicating that the isolates were PRCV. In a serological survey of 30 serum samples each collected from about 50 days old pigs in the 2 affected farms, 29 (97%) and 15 (50%) sera were positive for neutralizing antibody against the isolate with the titers ranging from 2 to 64, respectively.

【 授权许可】

Unknown