Development of an Enzyme-Linked Immunosorbent Assay Using Recombinant Chicken Anemia Virus Proteins Expressed in a Baculovirus Vector System

Journal of Veterinary Medical Science

Yosaburou OTAKI¹  Akira IWATA¹  Noriko IWATA¹  Miyuki FUJINO¹  Susumu UEDA¹  Kotaro TUCHIYA¹ 

[1]Nippon Institute for Biological Science

关键词: chicken anemia virus;    ELISA;    recombinant baculovirus;   

DOI  :  10.1292/jvms.60.175

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(10)Recombinant baculoviruses were constructed to express the putative proteins VP1, VP2 or VP3 of the chicken anemia virus (CAV). The recombinant VP1, VP2 or VP3 were detected by SDS-PAGE, and their molecular weights were 50, 30/27 and 16 kDa, respectively. The VP2 and VP3 reacted with sera from CAV-infected chickens in Western blot analysis and when used as an enzyme-linked immunosorbent assay (ELISA) antigen, but VP1 did not. Antibodies to CAV were detected, by ELISA using crude insect cell lysates containing VP2 or VP3, from 2 to 20 weeks or 2 to 7 weeks after CAV infection, respectively. These findings indicate that recombinant VP2 and VP3 expressed in the baculovirus vector system can be used as antigens to detect anti-CAV antibodies in ELISA.

【 授权许可】

Unknown