Gestational Changes in Production of NO and Expression of NOS mRNA Isoforms in the Rat Placenta

Journal of Veterinary Medical Science

Kazuaki TANAKA¹  Masako YAMAMOTO¹  Takehito SUZUKI¹  Hiroshi YOSHIKAWA¹  Hidetoshi MORITA¹  Tatsuya TAKIZAWA¹  Yoshinori IKEDA¹ 

[1] Graduate School of Veterinary Medicine, Azabu University

关键词: electron paramagnetic resonance (EPR);    fetal placenta;    nitric oxide;   

DOI  :  10.1292/jvms.71.495

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(6)To monitor changes in NO production over time in the fetal placenta of rats, we used electron paramagnetic resonance spectroscopy with the Fe-N-(dithiocarboxy) sarcosine (Fe-DTCS) complex as an NO-trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was examined in parallel using quantitative RT-PCR. NO production was first detected on day 13.5 of gestation. NO levels reached a peak on day 15.5, then decreased significantly during the last few days of gestation. The pattern of expression of NOS II mRNA was in good agreement with changes in NO levels, whereas NOS III mRNA expression did not change markedly during gestation. Thus, it appears that NO levels in the placenta are NOS II-dependent and differ at different gestational stages.

【 授权许可】

Unknown