Canonical Wnt Signaling Pathway Plays an Essential Role in N-Methyl-N-Nitrosurea Induced Gastric Tumorigenesis of Mice

Journal of Veterinary Medical Science

Hyoung-Chin KIM³  Kyoung-Sun LEE¹  Hyo-Jung KWON³  Dae-Yong KIM²  Jin-Hee SEO¹  Eui-Suk JEONG¹  Dong-Gu JEONG¹  Yang-Kyu CHOI¹  Seung-Ho HEO¹  Young-Suk WON³ 

[1] Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143–701, Republic of Korea;Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Republic of Korea;Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363–883, Republic of Korea

关键词: β-catenin;    canonical Wnt pathway;    gastric cancer;   

DOI  :  10.1292/jvms.12-0233

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Deregulated Wnt signaling pathway is implicated in many hereditary diseases and tumorigenesis including colorectal cancer, hepatocellular carcinoma and gastric cancer. In this study, to assess the relationship between chemically induced gastric tumor and canonical Wnt signaling pathway in genetically intact mice, histopathological and quantitative mRNA analyses were performed in C57BL/6J mice given drinking water containing N-methyl- N-nitrosurea (MNU). 60.5% of gastric adenoma and 27.9% of adenocarcinoma were observed 48 weeks after first administration. Also, in immunohistochemical analysis, aberrant expressions of phospho-GSK-3β, β-catenin, cyclin D1, c-Myc, osteopontin and COX-2 were found. In double immunofluorescent-antibody stains, β-catenin accumulation was colocalized with other proteins. mRNA levels of cyclin D1, c-myc and COX-2 were relatively higher in adenocarcinoma. Altogether, canonical Wnt pathway was highly involved in MNU induced gastric neoplasia of C57BL/6J mice, and it could be a considerably suitable system for the study to examine the linkage between gastric tumorigenesis and the canonical Wnt pathway.

【 授权许可】

Unknown