Mechanism of Macrophage Activation by Chitin Derivatives

Journal of Veterinary Medical Science

Masaaki MURAKAMI²  Masahiro OKUMURA¹  Toru FUJINAGA¹  Tsuyoshi KADOSAWA¹  Toshimitsu UEDE²  Takashi MORI¹ 

[1] Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Section of Immunopathogenesis, Institute of Immunological Science, Hokkaido University

关键词: apoptosis;    chitosan;    macrophage activation;    mannose receptor;    wound healing;   

DOI  :  10.1292/jvms.67.51

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(35)In order to analyze the detailed mechanisms responsible for macrophage activation by chitin derivatives, resident peritoneal macrophages were prepared and stimulated with chitin, chitosan and low-molecular weight chitosan. Our findings were as follows: (i) chitosan induced apoptosis of peritoneal macrophages, but this did not occur when chitin or water soluble low-molecular weight chitosan were used; (ii) chitosan treatment induced activation markers, such as the major histocompatibility complex (MHC) class I, class II, Fc receptors, transferrin receptor, mannose receptor, Fas, and macrophage inflammatory protein (MIP)-2, whereas chitin and low molecular weight soluble chitosan induced only the expression of MHC class I and II molecules; (iii) apoptosis induced by chitosan was mediated by the Fas signaling pathway, in response to phagocytosis via the mannose receptor. We conclude that since chitosan activates macrophages, this may be the mechanism by which it accelerates wound healing.

【 授权许可】

Unknown