Humoral immune response of European eel Anguilla anguilla experimentally infected with Anguillicola crassus

Diseases of Aquatic Organisms

K. Knopf¹  M. H. T. van der Heijden¹  H. Taraschewski¹  K. Naser¹ 

关键词: Anguilla anguilla;    Anguillicola crassus;    Humoral immune response;    Experimental infection;   

DOI  :  10.3354/dao042061

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A humoral immune response of the European eel Anguilla anguilla elicited by an experimental infection was demonstrated for the first time against the swimbladder nematode Anguillicola crassus. Eels were experimentally infected onceor repeatedly and the antibody response was observed over a period of 325 d. Specific antibodies against A. crassus in the peripheral blood of the eels were measured using an ELISA and the immunoblot technique. Anti-A. crassus antibodieswere first observed 8 wk post infection, and appeared to be independent of both the number of infective third stage larvae (L₃) administered and the frequency of administration. However, individual eels showed great differences in the course ofthe antibody response. The late appearance of antibodies in the peripheral blood supports the hypothesis that not the invading L₃ but rather the adult parasites elicit the production of specific antibodies. A stage-specific antibody responseagainst the L₃ was not observed. Main antigens are located in the body wall, especially in the gelatinous outer cuticle, of adult A. crassus.

【 授权许可】

Unknown