Intracellular Reactive Oxygen Species Production by Polymorphonuclear Leukocytes in Bovine Leukemia Virus-Infected Dairy Cows

Journal of Veterinary Medical Science

Andréia Oliveira LATORRE⁴  Fernando Nogueira de SOUZA²  Francisco Palma RENNO¹  Camila Freitas BATISTA²  Eduardo Milton RAMOS SANCHEZ³  Alice Maria Melville Paiva DELLA LIBERA²  Maiara Garcia BLAGITZ²  Rebeca Alves WEIGEL²  Maria Claudia Araripe SUCUPIRA² 

[1] Department of Nutrition and Animal Production, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo;Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo;Department of Immunology, Institute of Biomedical Sciences, University of São Paulo;Department of Pathology, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo

关键词: deltaretrovirus;    neutrophils;    oxidative burst;   

DOI  :  10.1292/jvms.11-0246

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(2)The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. aureus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.

【 授权许可】

Unknown