New Insights into HTLV-1 Particle Structure, Assembly, and Gag-Gag Interactions in Living Cells

Viruses

Keir H. Fogarty¹  Wei Zhang¹  Iwen F. Grigsby¹  Jolene L. Johnson¹  Yan Chen¹  Joachim D. Mueller¹ 

[1] ¹Institute for Molecular Virology, University of Minnesota, Minneapolis, 18-242 Moos Tower, 515 Delaware St. SE, Minneapolis, MN 55455, USA

关键词: deltaretrovirus;    lentivirus;    spectroscopy;    fluorescence;    tomography;   

DOI  :  10.3390/v3060770

来源: mdpi

【 摘 要 】

Human T-cell leukemia virus type 1 (HTLV-1) has a reputation for being extremely difficult to study in cell culture. The challenges in propagating HTLV-1 has prevented a rigorous analysis of how these viruses replicate in cells, including the detailed steps involved in virus assembly. The details for how retrovirus particle assembly occurs are poorly understood, even for other more tractable retroviral systems. Recent studies on HTLV-1 using state-of-the-art cryo-electron microscopy and fluorescence-based biophysical approaches explored questions related to HTLV-1 particle size, Gag stoichiometry in virions, and Gag-Gag interactions in living cells. These results provided new and exciting insights into fundamental aspects of HTLV-1 particle assembly—which are distinct from those of other retroviruses, including HIV-1. The application of these and other novel biophysical approaches promise to provide exciting new insights into HTLV-1 replication.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.