Concentrations and Specific Antibodies to Staphylococcal Enterotoxin-C and Toxic Shock Syndrome Toxin-1 in Bovine Mammary Gland Secretions, and Inflammatory Response to the Intramammary Inoculation of These Toxins

Journal of Veterinary Medical Science

Masashi ITAGAKI²  Kenzo KAI⁵  Katsuo KUMAGAI⁵  Jin KOBAYASHI¹  Shin-ichi KAMATA³  Toshinobu KUROISHI⁵  Minoru OHTA⁴  Ken-ichi KOMINE⁵ 

[1] Department of Animal Reproduction, Miyagi Agricultural College Research Farm;Okitama Veterinary Clinical Center, Yamagata Prefecture Federation Agricultural Mutual Aid Association;Department of Veterinary Hygiene, Nippon Veterinary and Animal Science University;Faculty of Veterinary Science, National University of La Plata;T Cell Research Institute In Bldg. ICR

关键词: intramammary inoculation;    specific antibody;    staphylococcal bovine mastitis;    staphylococcal enterotoxin-C;    toxic shock syndrome toxin-1;   

DOI  :  10.1292/jvms.65.899

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)Cited-By(12)To investigate the pathological role of staphylococcal enterotoxins (SEs) and toxic shock syndrome toxin-1 (TSST-1) in bovine mastitis, the production of SEs and TSST-1 was investigated in staphylococci isolated from 120 mammary gland secretions (MGS, 51 from no clinical sign-mammary glands and 69 from staphylococcal mastitic-mammary glands) collected from dairy farms where staphylococcal mastitis frequently occurred in Miyagi and Yamagata prefectures from 1997 to 1998. Concentrations of these toxins and specific antibody titers in each MGS were also measured. Furthermore, SEC and TSST-1 were inoculated into lactating mammary glands and inflammatory responses were analyzed. A high percentage of staphylococci including Staphylococcus aureus and coagulase-negative staphylococci isolated from both no clinical sign- and mastitic-MGS produced both SEC and/or TSST-1. The concentration of SEC increased with the severity of the mastitis, and was significantly higher (P<0.05) in acute mastitic-than in no clinical signs-MGS. Titers of specific antibodies to TSST-1 in MGS were significantly higher (P<0.05) than those to SEC, regardless of whether or not the cows were lactating or mastitic. Specific antibodies purified from MGS neutralized each toxin in vitro. A significant increase (P < 0.05) in somatic cell counts was induced by the intramammary inoculation of SEC but not TSST-1. These findings indicated that SEC rather than TSST-1 plays an important role in the pathology of staphylococcal bovine mastitis. The inflammatory activity of TSST-1 was probably neutralized by specific antibodies in MGS.

【 授权许可】

Unknown