Troglitazone Inhibits Vascular Endothelial Growth Factor–Induced Angiogenic Signaling via Suppression of Reactive Oxygen Species Production and Extracellular Signal–Regulated Kinase Phosphorylation in Endothelial Cells

Journal of Pharmacological Sciences

Jung-Ae Kim¹  Dinesh Thapa¹  Byung Chul Park¹  Su-Young Park¹  Jong Suk Lee¹ 

[1] College of Pharmacy, Yeungnam University, Korea

关键词: angiogenesis;    troglitazone;    matrix metalloproteinase (MMP);    reactive oxygen species (ROS);    extracellular signal–regulated kinase (ERK);   

DOI  :  10.1254/jphs.08305FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(54)Cited-By(11)Thiazolidinediones, peroxisome proliferators-activated receptor gamma (PPARγ) ligands, have been recognized as a potential therapeutic agents for the treatment of pathological neovascularization. In the present study, we examined the molecular mechanism by which troglitazone (TROG), a PPARγ agonist, exerts its inhibitory action in vascular endothelial growth factor (VEGF)-induced angiogenesis signaling. In an in vitro angiogenesis model using human umbilical vein endothelial cells, TROG (20 μM) significantly suppressed VEGF-induced cell proliferation and invasion of the cells into the Matrigel basement membrane, which was not reversed by treatment with PPAR antagonists, GW9662 (10 μM) and bisphenol A diglycidyl ether (10 μM). TROG also blocked VEGF-induced reactive oxygen species (ROS) production and its downstream extracellular signal–regulated kinase (ERK) phosphorylation, and this inhibitory effect was not reversed by GW9662 (10 μM). The antiangiogenic activity of TROG correlated with suppression of VEGF-induced matrix metalloproteinase (MMP)-2 and membrane type 1 (MT1)-MMP expression. In addition, the effects of TROG on VEGF-induced MMP-2 and MT1-MMP expression were comparable to those of the NADPH oxidase inhibitor diphenylene iodium (10 μM) and ERK inhibitor PD98056 (10 μM). Furthermore, in an in vivo angiogenesis system using a chick chorioallantoic membrane model, TROG dose-dependently inhibited VEGF-induced angiogenesis, which was similar to the inhibitory effect of N-acetylcysteine on VEGF-induced angiogenesis. The results suggest that the inhibitory effects of TROG on VEGF-induced angiogenesis were mediated through the suppression of VEGF-induced ROS production and ERK phosphorylation.

【 授权许可】

Unknown