White spot syndrome virus (WSSV) PCR-positive Artemia cysts yield PCR-negative nauplii that fail to transmit WSSV when fed to shrimp postlarvae

Diseases of Aquatic Organisms

Kuan-Fu Liu¹  Chu-Fang Lo¹  Yun-Shiang Chang¹  Shao-En Peng¹  Chung-Hsiung Wang¹  Guang-Hsiung Kou¹ 

关键词: White spot syndrome virus;    Brine shrimp;    Artemia;    Penaeus monodon;    WSSV diagnostic PCR;    Amplified restriction fragment length polymorphism;   

DOI  :  10.3354/dao049001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Positive results were obtained with nested white spot syndrome virus (WSSV) diagnostic PCR performed on 5 commercial brands of dry-packed Artemia cysts using several WSSV genomic sequence-specific primers. In 2 brands, PCR and nucleotidesequence analysis found CØT and CØG point mutations in the pms 146 WSSV amplicon, but in all 5 brands, the nucleotide sequences that were successfully amplified by the rr1, rr2 andtk-tmk gene-specific primer sets were identical to those of Penaeus monodon WSSV. However, despite the inarguable presence of WSSV or WSSV-like template DNA, we were unable to detect WSSV by PCR in hatched nauplii derived from PCR-positivecysts or in P. monodon postlarvae fed Artemia nauplii hatched from such cysts. Most simply, these results suggested that the cysts were externally contaminated with WSSV or WSSV-like template material that was removed during hatching andwashing of the nauplii. Given the small sequence variations found, it may also have been a variety of WSSV non-infectious for P. monodon or Artemia and derived from other crustaceans or arthropods in the Artemia environment. However,we could not establish this conclusively and a small possibility remained that the PCR template in these tests was derived from WSSV template present internally in the cysts and derived from infected Artemia adults. However small, this possibilitymust be vigorously tested, given the impact that a positive outcome could have on the shrimp industry.

【 授权许可】

Unknown