膜タンパク質予測に有用な膜貫通ヘリックスの特徴

Chem-Bio Informatics Journal

�? 敏之¹  美宅 成樹¹ 

[1] 名古屋大学工学研究科応用マテリアル理工学専攻

关键词: membrane protein;    膜タンパク質;    transmembrane helix;    膜貫通ヘリックス;    prediction;    予測;    bioinformatics;    バイオインフォマティクス;   

DOI  :  10.1273/cbij.4.110

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(39)Cited-By(4)For the development of a high-performance software system of membrane protein prediction, we analyzed the distribution of hydrophobicity and the amphiphilicity around transmembrane helices, using dataset of membrane proteins whose 3D structures or topologies are known.The moving average of 7 residues showed that there are a hydrophobicity peak in the center of a transmembrane helix and two amphiphilicity peaks at both ends of the hydrophobicity peak.The shapes of the peaks were asymmetric with respect to the center of the hydrophobicity peak.The membrane protein prediction system SOSUI (Hirokawa and Mitaku, Bioinformatics, 1998) was improved on the basis of the asymmetric profiles of hydrophobicity and amphiphilicity, resulting in the accuracy of 98% for positive dataset and 96% for negative one of prokaryotes, and the comparable accuracy was obtained also for eukaryotes.

【 授权许可】

Unknown