Analysis of Antibody Responses by Commercial Western Blot Assay in Horses with Alveolar Echinococcosis

Journal of Veterinary Medical Science

Nobuhiko KURODA¹  Kazue YAHAGI¹  Masanori KAWANAKA²  Toshihiko OHTAKI¹  Masahiro UENO¹ 

[1] Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan;Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan

关键词: echinococcosis;    horse;    PCR;    western blot;   

DOI  :  10.1292/jvms.11-0552

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(9)Cited-By(3)Commercial western blot (WB) assay was used to detect serum antibodies specific to Echinococcus multilocularis in 23 horses in which infection was confirmed by postmortem inspection at a slaughterhouse. Livers contained from 1 to >20 nodular lesions; foci diameter ranged from 1 to 25 mm. Antibody tests of serum from all 23 animals were negative for antigen bands at 7, 16, 18, and 26-28 kDa, which show specificity in the serum of human patients. However, sera from two infected horses with the largest nodules (diameter, 25 mm) showed positive response to one of the 22-kDa and 30-kDa antigen bands. It may be possible to diagnose E. multilocularis infection in horses based on the detection of these bands on commercial WB assay.

【 授权许可】

Unknown