Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland

Irish Veterinary Journal

JE Moore⁵  M Matsuda⁴  JS Elborn¹  JSG Dooley⁵  CE Goldsmith³  BC Millar³  T Buckley²  Y Maeda⁵  A Tazumi⁴ 

[1] Department of Respiratory Medicine, The Queen's University of Belfast, Respiratory Medicine, Belfast City Hospital, Lisburn Road, Belfast, Northern Ireland, BT9 7AB;Department of Equine Studies, University of Limerick, Ireland;Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland;Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagamihara 229-8501, Japan;School of Biomedical Sciences, Centre for Molecular Biosciences, University of Ulster, Coleraine, BT52 1SA, Northern Ireland

关键词: Pseudomonas aeruginosa;    PCR;    horse;    genotyping;    ERIC2 RAPD;    equine;   

Others  :  1149799
DOI  :  10.1186/2046-0481-62-7-456

【 摘 要 】

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.

   

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【 参考文献 】

• [1]Atherton JG, Pitt TL: Types of Pseudomonas aeruginosa isolated from horses. Eq Vet J 1982, 14(4):329-332.
• [2]Clarke L, Moore JE, Millar BC, et al.: Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland. Brit J Biomed Sci 2008, 65(1):18-21.
• [3]Grif K, Karch H, Schneider C, et al.: Comparative study of five different techniques for epidemiological typing of Escherichia coli O157. Diag Microbiol Infect Dis 1998, 32(3):165-176.
• [4]Gürtler V, Mayall BC: Genomic approaches to typing, taxonomy and evolution of bacterial isolates. Int J Sys Evol Microbiol 2001, 51(1):3-16.
• [5]Kurlenda J, Grinholc M, Jasek K, et al.: RAPD typing of methicillin-resistant Staphylococcus aureus: a seven-year experience in a Polish hospital. Med Sci Monitor 2007, 13(6):MT13-18.
• [6]Millar BC, Xu J, Moore JE: Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J Clin Microbiol 2002, 40(5):1575-1580.
• [7]Power EG: RAPD typing in microbiology - a technical review. J Hosp Infect 1996, 34(4):247-265.
• [8]Renders N, Romling Y, Verbrugh H, et al.: Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsedfield gel electrophoresis of DNA macrorestriction fragments. J Clin Microbiol 1996, 34(12):3190-3195.
• [9]Samper JC, Tibary A: Disease transmission in horses. Theriogenology 2006, 66(3):551-559.
• [10]Speert DP: Molecular epidemiology of Pseudomonas aeruginosa. Front Biosci 2002, 7:e354-361.
• [11]Tenover FC, Arbeit RD, Goering RV, et al.: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995, 33(9):2233-2239.