A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

BioTechniques

Robert N. Wilkinson¹  Stone Elworthy²  Philip W. Ingham²  Fredericus J.M. van Eeden² 

[1] 1Department of Cardiovascular Science, University of Sheffield, Medical School, Sheffield, United Kingdom;2Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom;

关键词: zebrafish;    embryo;    genotyping;    PCR;    fin clip;   

DOI  :  10.2144/000114116

来源: DOAJ

【 摘 要 】

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

【 授权许可】

Unknown