Molecular Cloning and Expression of Cytochrome P450 2D6 in the Livers of Domestic Cats

Journal of Veterinary Medical Science

Tetsuya KOMATSU¹  Kouichi HONDA¹  Takeo HIRAGA¹  Hiroki TERAOKA¹  Akira KUBOTA¹  Takio KITAZAWA¹ 

[1] School of Veterinary Medicine, Rakuno Gakuen University

关键词: carnivore;    cytochrome P450 2D;    domestic cats;    felid;    xenobiotics;   

DOI  :  10.1292/jvms.10-0150

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(4)Cytochrome P450 2D (CYP2D) is one of the major metabolizing enzymes for drugs in humans and other mammals. Although domestic cats (Felis catus) are the most common companion animal in many countries, the properties of their drug-metabolizing system are largely unknown. We determined the complete cDNA sequence of the CYP2D open reading frame (1,500 bp) with both 5'- and 3'-untranslated regions in domestic cats. Feline CYP2D consists of 500 deduced amino acids and shows high homology with CYP2Ds of primates and rodents, particularly with the canine CYP2D homologue, and was designated as CYP2D6 based on its genomic structure. CYP2D6 transcripts were expressed predominantly in the liver and to a much lesser in the testis, suggesting the role of xenobiotic metabolism in the liver.

【 授权许可】

Unknown