Evidence for the Presence of Keratinocyte Growth Factor (KGF) in Human Ovarian Follicles

Endocrine Journal

AKIHISA FUJIMOTO¹  MIKIO MOMOEDA¹  OSAMU TSUTSUMI¹  TOSHIHIRO FUJIWARA¹  TETSU YANO¹  AKIHIKO SUENAGA¹  HISAHIKO HIROI¹  KAORI KOGA¹  HIROTAKA MATSUMI¹  YUTAKA OSUGA¹  RYUGO OKAGAKI¹  KOJI KUGU¹  YUJI TAKETANI¹ 

[1] Department of Obstetrics and Gynecology, University of Tokyo

关键词: Ovary;    Keratinocyte growth factor;    Follicular fluid;    IVF-ET;   

DOI  :  10.1507/endocrj.48.161

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(16)Cited-By(2)The presence of keratinocyte growth factor (KGF) in human follicular fluid (FF) was investigated in a total of 145 FFs obtained during oocyte retrieval for in vitro fertilization (IVF) from 29 patients with no apparent endocrine disorders. The concentrations of KGF, estradiol, progesterone, testosterone and human chorionic gonadotropin (hCG) in FF were measured by enzyme-linked immunosorbent assay. FF samples contained relatively higher amounts of KGF (2194±87pg/ml), whereas its concentrations in serum were below assay limit (<31.2pg/ml). Concentrations of KGF in FF were positively correlated with both progesterone (r=0.311, p<0.0005) and testosterone (r=0.230, p<0.01) concentrations in FF. However, KGF concentrations were not ignificantly correlated with estradiol and hCG concentrations. KGF in FF was detected as a broad band (26-29kD) by immunoblotting, the size being reduced by 7kD after N-glycosidase treatment. In an in vitro experiment, KGF suppressed the basal and hCG-stimulated progesterone production by cultured human luteinized granulosa cells. In summary, we demonstrated the presence of KGF in human ovarian follicles, suggesting its possible role as a localfactor in regulating human ovarian functions.

【 授权许可】

Unknown