Erythrocyte-Replaced Mouse Model for Haemoparasite Studies: Comparison of NOD/shi-scid and C.B-17/Jcl-scid Mouse upon Acceptability of Human Erythrocytes

Journal of Veterinary Medical Science

Qiang WEI³  Aya ZAMOTO³  Ichiro AZUMA¹  Kyoji HIOKI²  Masayoshi TSUJI³  Chiaki ISHIHARA³ 

[1] Hakodate National College of Technology;Central Institute of Experimental Animals;School of Veterinary Medicine, Rakuno Gakuen University

关键词: Babesia;    NOD/shi-scid;    protozoan hemoparasite;    SCID mouse;    Theileria;   

DOI  :  10.1292/jvms.65.831

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(44)Cited-By(1)The erythrocyte-exchanged chimera mouse model has become to be a significant tool for studying animal and human (hu) protozoan haemoparasites, though the usefulness of this model varies depending primarily on the acceptability of xenogeneic red blood cells (RBC). To find a superior recipient in comparison with C.B-17/Jcl mouse with severe combined immuno-deficiency (scid) mutation, we examined in this report the non-obese diabetes (NOD)/shi-scid mouse, a recently available strain of SCID. When 2.5 × 108 of fluorescent dye-labeled hu-RBCs were transfused, C.B-17scid mouse eliminated them logarithmically by a simple linear regression, while NOD-scid mouse eradicated hu-RBCs by a unique two-step fashion, i.e., a potent but only briefly functioning RBC eradication followed by a weak steadily functioning step. The means of regression line constance ± their standard deviations (SD) of 205 C.B-17scid and of 213 NOD-scid mice for their short- and long-lasting steps were -0.73 ± 0.63, -0.53 ± 0.25 and -0.16 ± 0.10, respectively. Hu-RBC half-lives determined from these means of C.B-17scid mice and of NOD-scid mice for the short- and long-living steps were 3.6, 4.9 and 16.3 hr, respectively. Higher hu-RBC acceptability of NOD-scid mouse, especially at their long-lasting step, was also demonstrated under at an activated state of mouse innate immunity. Treatment with 1.0 mg heat-killed Candida cells caused an acceleration of hu-RBC elimination in both mouse strains but the magnitudes for the short- and long-living steps of NOD-scid mice evaluated by "stimulation index" were only 1/2.6 and 1/7.6 of C.B-17scid mice, respectively.

【 授权许可】

Unknown