Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil

Ciência Rural

Sampaio-Júnior, Francisco Dantas⁴  Oliveira, Wanessa Batista Lima²  Sousa, Paulo Geovani Silva⁴  Scofield, Alessandra⁴  Cunha-Santos, Rafaelle⁴  Monteiro, Silvia Gonzalez⁵  Mourão, Fábio Rodrigo Paixão¹  Góes-Cavalcante, Gustavo⁴  Gabriel, Áurea Martins³  Magalhães-Matos, Paulo Cesar⁴  Barros, Flávia de Nazaré Leite⁴ 

[1] Secretaria Municipal de Saúde do Município de Macapá, Macapá, Brasil;Independent biologist, Ananindeua, Brasil;Universidade Federal do Pará, Belém, Brazil;Universidade Federal do Pará, Castanhal, Brazil;Universidade Federal de Santa Maria, Santa Maria, Brazil

关键词: Trypanosoma evansi;    PCR;    Procyonidae;    Eastern Amazon;   

DOI  :  10.1590/0103-8478cr20150679

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crab-eating raccoons and captive and free-living coatis in Pará State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Pará State, being this the first such report in P. cancrivorus.

【 授权许可】

Unknown