Interactions of Organic Anion Transporters and Organic Cation Transporters With Mycotoxins

Journal of Pharmacological Sciences

Suparat Khamdang²  Michio Takeda²  Minoru Tsuda²  Rie Noshiro-Kofuji²  Naohiko Anzai²  Hitoshi Endou²  Kittipong Tachampa²  Surawat Jariyawat¹  Samaisukh Sophasan¹  Toshiyuki Fukutomi² 

[1] Department of Physiology, Faculty of Science, Mahidol University, Thailand;Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan

关键词: organic anion transporter;    organic cation transporter;    SLC22;    mycotoxin;    aflatoxin;   

DOI  :  10.1254/jphs.FP0070911

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(38)Cited-By(18)Mycotoxins are secondary metabolites of moulds that which exert adverse effects in humans and animals. It is known that direct cellular toxicity is often associated with increased cellular accumulation of toxic compounds, and membrane transport may be the first fundamental stage in the development of the cytotoxicity. To elucidate the entry pathway for mycotoxins into cells, we have investigated the interactions of human and rat organic anion transporters (hOATs/rOats) and human organic cation transporters (hOCTs) with mycotoxins using cells stably expressing hOATs/rOats/hOCTs. The mycotoxins tested were aflatoxin B1, α-zearalenol, citrinin, citrioveridine, cyclopiazonic acid, fumonisin B1, gliotoxin, patulin, penicillic acid, rubratoxin B, and zearalenone. These mycotoxins inhibited organic anion uptake mediated by hOAT1-4, and organic cation uptake mediated by hOCT1-2. By comparing the IC50 values of mycotoxins for hOATs, it was found that hOAT1 and hOAT3 exhibited higher affinity interactions with mycotoxins than hOAT2 and hOAT4. There was no interspecies difference between humans and rats for the interactions of OATs with mycotoxins except that of OAT3 with rubratoxin B. Finally, we observed that hOAT1-4 and hOCT1-2 mediated the uptake of aflatoxin B1. In conclusion, hOATs and hOCTs interacted with various mycotoxins. Considering the localization of hOATs/rOats and hOCTs, it was suggested that these transporters were the possible entrance pathway for mycotoxins in kidney and liver, leading to the induction of adverse effects in humans and rats.

【 授权许可】

Unknown