A search for Clostridium difficileribotypes 027 and 078 in Brazil

Brazilian Journal of Infectious Diseases

Rodrigues Filho, Edison Moraes²  Persson, Søren¹  Pires Neto, Renata³  Monteiro, Alexandre de Almeida³  Pasqualotto, Alessandro Comarú³ 

[1] Statens Serum Institut, Copenhagen, Denmark;Grupo Hospitalar Conceição, Porto Alegre, Brazil;Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Brazil

关键词: Multiplex-PCR;    Clostridium difficile;    HRM;    Toxin gene profiling;   

DOI  :  10.1016/j.bjid.2014.08.004

来源: Contexto

【 摘 要 】

Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.

【 授权许可】

Unknown