Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo | Science Publications

American Journal of Animal and Veterinary Sciences

Margarita Y. Mendoza-Ramos¹  Aurora Martinez-Romero¹  Joel Lopez-Perez¹  Jose R. Hernandez-Salgado¹  Raul Villegas-Viscaino¹  Miguel Arenas-Vargas¹  Rafael Rodriguez-Martinez¹  Norma Urtiz-Estrada¹  Maribel Cervantes-Flores¹  Jose L. Ortega-Sanchez¹ 

关键词: M. bovis-BCG vaccine;    1;    25 dihydroxyvitamin;    nitrites;    nitrates;    goats;   

DOI  :  10.3844/ajavsp.2009.6.13

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO_2^- and NO_3^-

【 授权许可】

Unknown