Isolation and characterization of canine tumor endothelialcells

Journal of Veterinary Medical Science

Yusuke IZUMI¹  Kenji HOSOYA¹  Masahiro OKUMURA¹  Satoshi TAKAGI²  Yuki HOSHINO² 

[1]Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060�?0818, Japan

[2]Veterinary Teaching Hospital, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060�?0818, Japan

关键词: angiogenesis;    canine;    tumor endothelial cell;   

DOI  :  10.1292/jvms.14-0347

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)The present study involved the isolation and characterization of canine tumor endothelialcells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting followingFITC labeling with UEA1 lectin, and they were characterized by measuring genetic andhistopathological endothelial markers. Isolated TECs exhibited a cobblestone-likemorphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) andVon Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminomaexhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7expression was not detected in TECs and tumor cells derived from a hepatocellularcarcinoma. Understanding TEC specific traits may be important in the development of moreefficacious anti-angiogenic therapies that do not induce adverse effects.

【 授权许可】

Unknown