Estimation of bacterial use of dissolved organic nitrogen compounds in aquatic ecosystems using Biolog plates

Aquatic Microbial Ecology

M. Montserrat Sala¹  Jarone Pinhassi Josep M. Gasol¹ 

关键词: Biolog;    Bacterioplankton;    Substrate utilization;    Dissolved organic nitrogen;    DON;    Nutrients;    Amino acids;   

DOI  :  10.3354/ame042001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: We demonstrated that bacterioplankton utilization of N-containing substrates, in relation to overall substrate utilization, can be assessed by calculating a nitrogen use (NUSE) index from Biolog-ECO microplate readings. The NUSE index was positively correlated with bacterial-specific aminopeptidase activity in coastal plankton samples, and it decreased after ammonia or amino acid addition to seawater cultures; this indicated that the index was sensitive to changes in N concentrations in natural samples. The index provides valuable information on dissolved organic nitrogen processing by natural bacterial assemblages, and thereby substantially expands the utility of the Biolog-ECO microplates.

【 授权许可】

Unknown